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b16f1 mouse melanoma cell line (ATCC)

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ATCC b16f1 mouse melanoma cell line
B16f1 Mouse Melanoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b16f1+mouse+melanoma+cell+line/B16-F1/pm41863466-43-1-10
Average 97 stars, based on 1194 article reviews

b16f1 mouse melanoma cell line - by Bioz Stars, 2026-09

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Article Title: Lineage-restricted regulation of SCD and fatty acid saturation by MITF controls melanoma phenotypic plasticity
Article Snippet: B16F1 mouse melanoma cell line , Obtained from ATCC , ATCC CRL-6323.

Article Title: Coactivation of innate immune suppressive cells induces acquired resistance against combined TLR agonism and PD-1 blockade.
Article Snippet: Immune checkpoint blockade therapy has been successfully applied in clinical settings as a standard therapy for many cancer types, but its clinical efficacy is restricted to patients with immunologically hot tumors.. Various strategies to modify the tumor microenvironment (TME), such as Tolllike receptor (TLR) agonists that can stimulate innate immunity, have been explored but have not been successful.. Here, we show a mechanism of acquired resistance to combination treatment consisting of an agonist for multiple TLRs, OK432 (Picibanil), and programmed cell death protein 1 (PD1) blockade.

Cell Culture:

Article Title: Rac1 mediates dendrite formation in response to melanocyte stimulating hormone and ultraviolet light in a murine melanoma model.
Article Snippet: .. Cells and cell culture The B16F1 mouse melanoma cell line was obtained from American Type Culture Collection (Rockville, MD). .. The cells were checked periodically for contamination with mycoplasma by staining with bisBenzimide Hoechst trichloride (Sigma, St. Louis, MO).

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Inflammatory profiles of AT-3 mammary tumors and B16F1 melanoma. AT-3 and B16F1 tumors were grown in C57BL/6 mice for 21 days and 14 days, respectively. (A) Comparative heatmap showing cytokines and chemokines expressed in AT-3 and B16F1 tumors using Proteome Profiler array ( n = 4 tumors, p < 0.05). (B) Representative images and quantification of CD45 staining in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors, each dot represents the mean value calculated from 5 random images taken with a 10x objective per tumor. (C) Comparison of granzyme B levels in AT-3 and B16F1 tumor extracts. ( n = 32 AT-3 and 25 B16F1 tumors). (D) Scatter plot of Spearman’s rank correlation between tumor weight and granzyme B in AT-3 ( n = 32) and B16F1 tumors ( n = 25). (E) Comparison of PD-L1 levels in AT-3 ( n = 19) and B16F1 tumors ( n = 14)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The localization, origin, and impact of platelets in the tumor microenvironment are tumor type-dependent

doi: 10.1186/s13046-024-03001-2

Figure Lengend Snippet: Inflammatory profiles of AT-3 mammary tumors and B16F1 melanoma. AT-3 and B16F1 tumors were grown in C57BL/6 mice for 21 days and 14 days, respectively. (A) Comparative heatmap showing cytokines and chemokines expressed in AT-3 and B16F1 tumors using Proteome Profiler array ( n = 4 tumors, p < 0.05). (B) Representative images and quantification of CD45 staining in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors, each dot represents the mean value calculated from 5 random images taken with a 10x objective per tumor. (C) Comparison of granzyme B levels in AT-3 and B16F1 tumor extracts. ( n = 32 AT-3 and 25 B16F1 tumors). (D) Scatter plot of Spearman’s rank correlation between tumor weight and granzyme B in AT-3 ( n = 32) and B16F1 tumors ( n = 25). (E) Comparison of PD-L1 levels in AT-3 ( n = 19) and B16F1 tumors ( n = 14)

Article Snippet: The mouse B16F1 melanoma cell line characterized by a low metastatic potential [ ], was purchased from ATCC.

Techniques: Staining, Comparison

Angiogenic profiles of AT-3 mammary tumors and B16F1 melanoma. (A) Comparative heatmap of angiogenic factors expressed in AT-3 and B16F1 tumor extracts using Proteome Profiler array ( n = 4 tumors, p < 0.05). (B) Representative images of collagen IV staining in AT-3 and B16F1 tumors. (C) Quantification of blood vessels identified by collagen IV staining in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors, each dot represents the mean value calculated from 5 random images taken with a 10x objective per tumor. (D) Quantification of mean vessel perimeter in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors; each dot represents the mean value calculated from 10 random images taken with a 10x objective per tumor. (E) Tumor vascular perfusion as assessed by measurement of intratumor FITC-dextran content of B16F1 and AT-3 tumors explanted 5 min after intravenous injection of FITC-dextran, 150 kDa. ( n = 27 AT-3 and 20 B16F1 tumors). (F) H&E staining of both tumor types showing tumor bleeding only in B16F1 tumors. Arrows indicate intratumor hemorrhage. Comparison of intratumor hemoglobin content between AT-3 ( n = 32) and B16F1 tumors ( n = 25). ns, non-significant

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The localization, origin, and impact of platelets in the tumor microenvironment are tumor type-dependent

doi: 10.1186/s13046-024-03001-2

Figure Lengend Snippet: Angiogenic profiles of AT-3 mammary tumors and B16F1 melanoma. (A) Comparative heatmap of angiogenic factors expressed in AT-3 and B16F1 tumor extracts using Proteome Profiler array ( n = 4 tumors, p < 0.05). (B) Representative images of collagen IV staining in AT-3 and B16F1 tumors. (C) Quantification of blood vessels identified by collagen IV staining in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors, each dot represents the mean value calculated from 5 random images taken with a 10x objective per tumor. (D) Quantification of mean vessel perimeter in AT-3 and B16F1 tumors. n = 8 AT-3 and 6 B16F1 tumors; each dot represents the mean value calculated from 10 random images taken with a 10x objective per tumor. (E) Tumor vascular perfusion as assessed by measurement of intratumor FITC-dextran content of B16F1 and AT-3 tumors explanted 5 min after intravenous injection of FITC-dextran, 150 kDa. ( n = 27 AT-3 and 20 B16F1 tumors). (F) H&E staining of both tumor types showing tumor bleeding only in B16F1 tumors. Arrows indicate intratumor hemorrhage. Comparison of intratumor hemoglobin content between AT-3 ( n = 32) and B16F1 tumors ( n = 25). ns, non-significant

Article Snippet: The mouse B16F1 melanoma cell line characterized by a low metastatic potential [ ], was purchased from ATCC.

Techniques: Staining, Injection, Comparison

Continuous presence of intravascular and firmly adherent platelets in AT-3 mammary tumors and B16F1 melanoma. AT-3 or B16F1 cells were injected subcutaneously in the dorsal skin and allowed to grow for 5 to 7 days before surgical implantation of a dorsal skinfold chamber. Representative intravital images of the microcirculation of B16F1 and AT-3 tumors in mice injected with FITC-dextran and fluorescent antibodies to GPIX, fibrin, Gr-1, or PECAM-1, as indicated. White arrow indicates individual platelets adhering firmly to tumor vessels. The asterisk indicates a platelet-fibrin deposit. The white arrowhead shows a platelet-neutrophil complex adhering to tumor vessels. Bar = 10μm

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The localization, origin, and impact of platelets in the tumor microenvironment are tumor type-dependent

doi: 10.1186/s13046-024-03001-2

Figure Lengend Snippet: Continuous presence of intravascular and firmly adherent platelets in AT-3 mammary tumors and B16F1 melanoma. AT-3 or B16F1 cells were injected subcutaneously in the dorsal skin and allowed to grow for 5 to 7 days before surgical implantation of a dorsal skinfold chamber. Representative intravital images of the microcirculation of B16F1 and AT-3 tumors in mice injected with FITC-dextran and fluorescent antibodies to GPIX, fibrin, Gr-1, or PECAM-1, as indicated. White arrow indicates individual platelets adhering firmly to tumor vessels. The asterisk indicates a platelet-fibrin deposit. The white arrowhead shows a platelet-neutrophil complex adhering to tumor vessels. Bar = 10μm

Article Snippet: The mouse B16F1 melanoma cell line characterized by a low metastatic potential [ ], was purchased from ATCC.

Techniques: Injection

Impact of chronic severe thrombocytopenia on proliferation and apoptosis in B16F1 and AT-3 tumors. (A) Schematic representation of severe chronic thrombocytopenia induction. Starting from the day of B16F1 and AT-3 tumor cell implantation, c-mpl-deficient mice were injected every 5 days with a platelet-depleting polyclonal antibody at low dose (0.5 µg/g mouse). Littermate control mice were injected with a non-immune IgG. (B) Wet weight of B16F1 tumors (platelet 100%, n = 27; <10%, n = 24) and AT-3 tumors (platelet 100%, n = 28; <10%, n = 32). (C) Quantification of KI67-positive cells in B16F1 tumors and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia. n = 7 (100%) and 9 (< 10%) different B16F1 tumors; and n = 7 (100%) and 8 (< 10%) different AT-3 tumors. (D) Quantification of TUNEL-positive cells in B16F1 tumors and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia mice. n = 7 (100%) and 9 (< 10%) different B16F1 tumors; and n = 5 (100%) and 7 (< 10%) different AT-3 tumors. ns: non-significant

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The localization, origin, and impact of platelets in the tumor microenvironment are tumor type-dependent

doi: 10.1186/s13046-024-03001-2

Figure Lengend Snippet: Impact of chronic severe thrombocytopenia on proliferation and apoptosis in B16F1 and AT-3 tumors. (A) Schematic representation of severe chronic thrombocytopenia induction. Starting from the day of B16F1 and AT-3 tumor cell implantation, c-mpl-deficient mice were injected every 5 days with a platelet-depleting polyclonal antibody at low dose (0.5 µg/g mouse). Littermate control mice were injected with a non-immune IgG. (B) Wet weight of B16F1 tumors (platelet 100%, n = 27; <10%, n = 24) and AT-3 tumors (platelet 100%, n = 28; <10%, n = 32). (C) Quantification of KI67-positive cells in B16F1 tumors and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia. n = 7 (100%) and 9 (< 10%) different B16F1 tumors; and n = 7 (100%) and 8 (< 10%) different AT-3 tumors. (D) Quantification of TUNEL-positive cells in B16F1 tumors and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia mice. n = 7 (100%) and 9 (< 10%) different B16F1 tumors; and n = 5 (100%) and 7 (< 10%) different AT-3 tumors. ns: non-significant

Article Snippet: The mouse B16F1 melanoma cell line characterized by a low metastatic potential [ ], was purchased from ATCC.

Techniques: Injection, Control, TUNEL Assay

Impact of thrombocytopenia on vascular permeability and endothelial integrity in B16F1 and AT-3 tumors. A. Representative images of the macroscopic aspects of B16F1 and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia, with or without intravenous injection of FITC-dextran, 2000 kDa prior to sacrifice. AT-3 tumors in control mice are highlighted by white arrows. B-C. Representative images and corresponding endothelial integrity scores of PECAM-1 ( B ) and VE-cadherin ( C ) staining for evaluation of endothelial integrity in AT-3 and B16F1 tumors from control mice and mice with chronic severe thrombocytopenia. Maximal intensity projections of optical sections are shown. D. Comparison of soluble PECAM-1 levels in plasma from control mice and mice with chronic severe thrombocytopenia with AT-3 (platelet 100%, n = 8; <10%, n = 5) or B16F1 (platelet 100%, n = 21; <10%, n = 12) tumors. ns: non-significant

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The localization, origin, and impact of platelets in the tumor microenvironment are tumor type-dependent

doi: 10.1186/s13046-024-03001-2

Figure Lengend Snippet: Impact of thrombocytopenia on vascular permeability and endothelial integrity in B16F1 and AT-3 tumors. A. Representative images of the macroscopic aspects of B16F1 and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia, with or without intravenous injection of FITC-dextran, 2000 kDa prior to sacrifice. AT-3 tumors in control mice are highlighted by white arrows. B-C. Representative images and corresponding endothelial integrity scores of PECAM-1 ( B ) and VE-cadherin ( C ) staining for evaluation of endothelial integrity in AT-3 and B16F1 tumors from control mice and mice with chronic severe thrombocytopenia. Maximal intensity projections of optical sections are shown. D. Comparison of soluble PECAM-1 levels in plasma from control mice and mice with chronic severe thrombocytopenia with AT-3 (platelet 100%, n = 8; <10%, n = 5) or B16F1 (platelet 100%, n = 21; <10%, n = 12) tumors. ns: non-significant

Article Snippet: The mouse B16F1 melanoma cell line characterized by a low metastatic potential [ ], was purchased from ATCC.

Techniques: Permeability, Control, Injection, Staining, Comparison, Clinical Proteomics

Impact of chronic severe thrombocytopenia on the inflammatory and immune profiles of B16F1 and AT-3 tumors. A-B. Comparative heatmap of tumor cytokines and angiogenic factors expressed in ( A ) B16F1 and ( B ) AT-3 tumor extracts from control mice and mice with chronic severe thrombocytopenia mice using Proteome Profiler arrays ( n = 4 tumors per group, p < 0.05). C. Comparison of myeloperoxidase (MPO) levels used as a marker of neutrophil infiltration in B16F1 and AT-3 tumor extracts from control mice and mice with chronic severe thrombocytopenia. n = 25 (100%) and 22 (< 10%) B16F1 tumors; n = 24 (100%) and 29 (< 10%) AT-3 tumors. D. Quantification of CD45-positive cells in B16F1 and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia. n = 9 (100%) and 10 (< 10%) different B16F1 tumors; and n = 9 (100%) and 8 (< 10%) different AT-3 tumors. E. Comparison of cytotoxic cell infiltration among control mice and mice with chronic severe thrombocytopenia in B16F1 and AT-3 tumors by measurement of their granzyme B content. n = 25 (100%) and 22 (< 10%) different B16F1 tumors; and n = 20 (100%) and 30 (< 10%) different AT-3 tumors. ns: non-significant

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The localization, origin, and impact of platelets in the tumor microenvironment are tumor type-dependent

doi: 10.1186/s13046-024-03001-2

Figure Lengend Snippet: Impact of chronic severe thrombocytopenia on the inflammatory and immune profiles of B16F1 and AT-3 tumors. A-B. Comparative heatmap of tumor cytokines and angiogenic factors expressed in ( A ) B16F1 and ( B ) AT-3 tumor extracts from control mice and mice with chronic severe thrombocytopenia mice using Proteome Profiler arrays ( n = 4 tumors per group, p < 0.05). C. Comparison of myeloperoxidase (MPO) levels used as a marker of neutrophil infiltration in B16F1 and AT-3 tumor extracts from control mice and mice with chronic severe thrombocytopenia. n = 25 (100%) and 22 (< 10%) B16F1 tumors; n = 24 (100%) and 29 (< 10%) AT-3 tumors. D. Quantification of CD45-positive cells in B16F1 and AT-3 tumors from control mice and mice with chronic severe thrombocytopenia. n = 9 (100%) and 10 (< 10%) different B16F1 tumors; and n = 9 (100%) and 8 (< 10%) different AT-3 tumors. E. Comparison of cytotoxic cell infiltration among control mice and mice with chronic severe thrombocytopenia in B16F1 and AT-3 tumors by measurement of their granzyme B content. n = 25 (100%) and 22 (< 10%) different B16F1 tumors; and n = 20 (100%) and 30 (< 10%) different AT-3 tumors. ns: non-significant

Article Snippet: The mouse B16F1 melanoma cell line characterized by a low metastatic potential [ ], was purchased from ATCC.

Techniques: Control, Comparison, Marker

Cell survival of (A) CHO and (B) B16F1 cells at different cisplatin concentrations determined by the clonogenic assay for non-electroporated (non-EP) cells (black circles) and cells electroporated with 25 x 400 ns pulses at 3.9 kV/cm, 10 Hz repetition rate (dark blue squares), 1 × 200 ns pulse at 12.6 kV/cm (light blue diamonds) or 8 × 100 μs pulses at 1.1 (CHO) or 0.9 (B16F1) kV/cm, 1 Hz pulse repetition rate (orange triangles). Bars represent standard deviation, asterisks (*) show statistically significant differences (p < 0.05) to the survival of non-electroporated cells without cisplatin. Survival data were combined from the previous 8 (for non-electroporated cells and cells electroporated with 25 × 400 ns and 8 × 100 μs pulses) and the present study (for B16F1 cells, additional non-electroporated CHO cells and CHO cells electroporated with 1 × 200 ns pulse).

Journal: Radiology and Oncology

Article Title: Nanosecond Electric Pulses are Equally Effective in Electrochemotherapy with Cisplatin as Microsecond Pulses

doi: 10.2478/raon-2022-0028

Figure Lengend Snippet: Cell survival of (A) CHO and (B) B16F1 cells at different cisplatin concentrations determined by the clonogenic assay for non-electroporated (non-EP) cells (black circles) and cells electroporated with 25 x 400 ns pulses at 3.9 kV/cm, 10 Hz repetition rate (dark blue squares), 1 × 200 ns pulse at 12.6 kV/cm (light blue diamonds) or 8 × 100 μs pulses at 1.1 (CHO) or 0.9 (B16F1) kV/cm, 1 Hz pulse repetition rate (orange triangles). Bars represent standard deviation, asterisks (*) show statistically significant differences (p < 0.05) to the survival of non-electroporated cells without cisplatin. Survival data were combined from the previous 8 (for non-electroporated cells and cells electroporated with 25 × 400 ns and 8 × 100 μs pulses) and the present study (for B16F1 cells, additional non-electroporated CHO cells and CHO cells electroporated with 1 × 200 ns pulse).

Article Snippet: Mouse skin melanoma cell line B16F1 (European Collection of Authenticated Cell Cultures, cat. no. 92101203, Sigma Aldrich, Germany, mycoplasma free) was cultured in the same way as CHO cells except that Dulbecco’s Modified Eagle Medium (DMEM, cat. no. D5671, Sigma-Aldrich, Missouri, United States) supplemented with 10% FBS (cat. no. F9665, Sigma-Aldrich), 2.0 mM L-glutamine, 1 U/ml penicillin/ streptomycin and 50 μg/ml gentamycin was used instead of Nutrient Mixture F-12 Ham.

Techniques: Clonogenic Assay, Standard Deviation

Pt amount in cell pellets of (A) CHO and (B) B16F1 cells after 25 min incubation at different extracellular cisplatin concentrations in non-electroporated (non-EP) cells (black circles) and cells electroporated with 25 x 400 ns pulses at 3.9 kV/ cm, 10 Hz repetition rate (dark blue squares), 1 × 200 ns pulse at 12.6 kV/cm (light blue diamonds) or 8 × 100 μs pulses at 1.1 (CHO) or 0.9 (B16F1) kV/cm, 1 Hz pulse repetition rate (orange triangles). Bars represent standard deviation, asterisks (*) show statistically significant differences (p < 0.05) to the measured number of cisplatin molecules in non-electroporated cells at the same extracellular cisplatin concentration.

Journal: Radiology and Oncology

Article Title: Nanosecond Electric Pulses are Equally Effective in Electrochemotherapy with Cisplatin as Microsecond Pulses

doi: 10.2478/raon-2022-0028

Figure Lengend Snippet: Pt amount in cell pellets of (A) CHO and (B) B16F1 cells after 25 min incubation at different extracellular cisplatin concentrations in non-electroporated (non-EP) cells (black circles) and cells electroporated with 25 x 400 ns pulses at 3.9 kV/ cm, 10 Hz repetition rate (dark blue squares), 1 × 200 ns pulse at 12.6 kV/cm (light blue diamonds) or 8 × 100 μs pulses at 1.1 (CHO) or 0.9 (B16F1) kV/cm, 1 Hz pulse repetition rate (orange triangles). Bars represent standard deviation, asterisks (*) show statistically significant differences (p < 0.05) to the measured number of cisplatin molecules in non-electroporated cells at the same extracellular cisplatin concentration.

Techniques: Incubation, Standard Deviation, Concentration Assay

Cell survival as a function of the number of cisplatin molecules per cell for (A) CHO cells and (B) B16F1 cells in non-electroporated (non-EP) cells (black circles) and cells electroporated with 25 x 400 ns pulses at 3.9 kV/cm, 10 Hz repetition rate (dark blue squares), 1 × 200 ns pulse at 12.6 kV/cm (light blue diamonds) or 8 × 100 μs pulses at 1.1 (CHO) or 0.9 (B16F1) kV/cm, 1 Hz pulse repetition rate (orange triangles). Bars represent standard deviation. Survival data were combined from the previous (for non-electroporated CHO cells and CHO cells electroporated with 25 × 400 ns and 8 × 100 μs pulses) and the present study (for B16F1 cells, additional non-electroporated CHO cells and CHO cells electroporated with 1 × 200 ns pulse).

Journal: Radiology and Oncology

Article Title: Nanosecond Electric Pulses are Equally Effective in Electrochemotherapy with Cisplatin as Microsecond Pulses

doi: 10.2478/raon-2022-0028

Figure Lengend Snippet: Cell survival as a function of the number of cisplatin molecules per cell for (A) CHO cells and (B) B16F1 cells in non-electroporated (non-EP) cells (black circles) and cells electroporated with 25 x 400 ns pulses at 3.9 kV/cm, 10 Hz repetition rate (dark blue squares), 1 × 200 ns pulse at 12.6 kV/cm (light blue diamonds) or 8 × 100 μs pulses at 1.1 (CHO) or 0.9 (B16F1) kV/cm, 1 Hz pulse repetition rate (orange triangles). Bars represent standard deviation. Survival data were combined from the previous (for non-electroporated CHO cells and CHO cells electroporated with 25 × 400 ns and 8 × 100 μs pulses) and the present study (for B16F1 cells, additional non-electroporated CHO cells and CHO cells electroporated with 1 × 200 ns pulse).

Techniques: Standard Deviation

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